IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ANTIGEN PRESENTING CELLS ARE MODULATED BY HISTAMINE VIA H3/4R IN A MOUSE MODEL OF DERMATITIS
Autor/es:
CORONEL, VALENTÍN; VERMEULEN, MÓNICA; ALCAIN, JULIETA; SALAMONE, GABRIELA; TODERO, FLORENCIA; REARTE, BÁRBARA
Lugar:
Tucumán
Reunión:
Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019
Institución organizadora:
SAI
Resumen:
Allergic dermatitis (AD) is a T cell-mediated response to skin sensitizers. Both CD8+ and CD4+ Tcells determine the magnitude and duration of the response. Histamine (HIS) modulates thedevelopment of allergy, vascular permeability, itch and muscle constriction. Here, our focus ison HIS action on dendritic cells and the HIS receptors (HR) involved. A mouse model of AD wasdeveloped by topical treatment with the hapten DNFB (0.3% in acetone: water) for 5 days. Onday 1, we inoculated 1x105 bone marrow-derived dendritic cells (BMDC) treated with orwithout the antagonists: Thioperamide (Thio, 107M) for H3R and JNJ7777120 (JNJ, 107M) forH4R. On day 8, animals were euthanized and ears, lymph nodes and serum were collected forELISA, flow cytometry, proliferation assays and histopathology. Ear induration is increased inmice treated with DNFB and reduced when HR are inhibited (p