Role of IL10 on expansion of myeloid cells during immunosuppression induced by bacterial endotoxin

DANIELA, MONTAGNA; ALCAIN JULIETA; TODERO MARIA FLORENCIA; BÁRBARA REARTE

Tucuman

Congreso; SAI; 2019

In sepsis caused by gram-negative infections membrane lipopolysacharide (LPS) induces a pro-inflammatory state followed by an anti-inflammatory state that leads to immunosuppression (IS) where IL-10 has a relevant role. Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of immature cells which expands in these pathological conditions. The aim of this study was to evaluate phenotypic and functional aspects at the cellular level that allow us to understand the alterations that occur during IS processes. For this we use BALB / c wild type (WT) mice and mice deficient in IL-10 (KO). Immunosuppression model: daily inoculation of LPS in increasing concentrations. Population determinations: flow cytometry. Respiratory burst: DHR. Proliferation assays: CFSE. In peripheral blood KOIS mice showed a significant increase in myeloid cells with respect to KO mice, this same pattern is observed in granulocyte type MDSC (CD11b+Ly6G+Ly6C+ (N° abs): KO = 1.9x108 ± 5.7x107; KOIS = 3.2x108 ± 1.6x108, p