IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Renal endothelial cells stimulated by Shiga toxin 2 induce the secretion of perforin by gamma/delta T lymphocytes
Autor/es:
ROSSO, DAVID A; AMARAL, MARÍA MARTA; IBARRA, CRISTINA; ALVAREZ, ROMINA S; JANCIC, CAROLINA C
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; 67 Reunión Anual de la Sociedad Argentina de Inmunología; 2019
Institución organizadora:
SOCIEDAD ARGENTINA DE INMUNOLOGIA
Resumen:
gamma/delta T cells constitute a functionally specialized subset of T lymphocytes which act as early sensors of cellular stress and infection. The hemolytic uremic syndrome (HUS) associated with diarrhea, a consequence of Shiga toxin (Stx)-producing Escherichia coli infection, is a common cause of pediatric acute renal failure in Argentina. Stx type 2 (Stx2) causes direct damage to renal cells and induces local inflammatory responses which involved secretion of inflammatory mediators and the recruitment of innate immune cells. Previously, we demonstrated that gamma/delta T lymphocytes reduced the viability of human glomerular endothelial cells (HGEC) treated with Stx2. In this work, we aimed to investigate the mechanism by which gamma/delta T cells prompt the mortality of HGEC. To this purpose, we analyzed by flow cytometry, the expression of perforin in gamma/delta T cells after incubation with supernatants obtained from HGEC treated with Stx2 (0.001-1 ng/ml). Also, we evaluated by confocal microscopy, the interaction between gamma/delta T cells and HGEC treated or not with Stx2 (0.01 ng/ml). We observed that perforin expression was diminished when gamma/delta T cells were cultured with Stx2-stimulated HGEC supernatants (p