IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Platelets promote B. abortus monocyte invasion by establishing complexes with monocytes
Autor/es:
MILILLO, M. AYELEN; GIAMBARTOLOMEI, GUILLERMO H.; VELÁSQUEZ, LIS N.; RODRÍGUEZ, ANA M.; BARRIONUEVO, PAULA; TROTTA, ALDANA; DELPINO, M. VICTORIA; POZNER, ROBERTO G.
Lugar:
Cancún
Reunión:
Congreso; XII Congress of the Latin American Association of Immunology - ALAI/ XXIII Congress of the Mexican Society of Immunology - SMI; 2018
Institución organizadora:
ALAI y SMI
Resumen:
Brucellosis is an infectious disease elicited by bacteria of the genus Brucella. Platelets have been extensively described as mediators of hemostasis and responsible for maintaining vascular integrity. Nevertheless, they have been recently involved in the modulation of innate and adaptive immune responses. Although many interactions have been described between B. abortus and monocytes/macrophages, the role of platelets during monocyte/macrophage infection by these bacteria remained unknown. The aim of this study was to investigate the role of platelets in the immune response against B. abortus. We first focused on the possible interactions between B. abortus and platelets. To assess this, human platelets were co-incubated with GFP?B. abortus for 4 h and the interaction was quantified by confocal microscopy and flow cytometry. Our results showed that platelets were able to interact with bacteria in a dose-dependent fashion. Moreover, this interaction triggered platelet activation, measured as fibrinogen binding and P-selectin expression (p