MODULATION OF SPECIFIC TH22 RESPONSE BY MDR M. TUBERCULOSIS ISOLATED IN ARGENTINA

IMPERIALE BELÉN; GRAMBLICKA GEORGINA; GONZALEZ MONTANER PABLO; SASIAIN MARÍA DEL CARMEM; GARCÍA ANA; MORACHO LUCÍA; MORCILLO NORA; FERREYRA COMPAGNUCCI MALENA; MATTEO MARIO; PALMERO DOMINGO; DE LA BARRERA SILVIA

Mar del Plata

Congreso; Reunión conjunta SAIC SAI SAFIS 2018; 2018

Sociedad Argentina de Inmunología y Sociedad Argentina de Investigación Clínica y SAFIS

mans.Innate and adaptive immune responses are needed to controlM. tuberculosis (Mtb) infection. Although TH1 T lymphocytes (LT)are essential to control Mtb growth, other LT, such as TH22 wouldparticipate in this response. Previously we demonstrated that thelocal multidrug-resistant (MDR) Mtb strains M and Ra differentiallymodulated TH1 and TH17 responses. So, the aim of this study wasto explore the TH22 response induced by these strains.Peripheral blood from 25 MDR-TB, 24 fully drug-susceptible (S-TB)patients and from healthy donors (HD) were evaluated. Epidemiologicaland bacteriological data of patients were collected. PBMCswere cultured alone or with M, Ra or H37Rv γ irradiated strains (2:1Mtb to PBMC ratio) for 6 days. Then, cells were tested for CD4,CD8, IL-17 and IL-22 expression by FACS and IL-22 amounts weredetermined in cultured-supernatants by ELISA. IL-22 secretion fromMtb-stimulated PBMC was higher in MDR-TB and S-TB patientsthan HD (p