Decidualized cells respond differentially contributing to a tolerogenic microenviroment accordingly blastocyst quality

FERNÁNDEZ L, SOCZEWSKI E, GORI MS, SABBIONE F, VOTA D, PAPARINI D, MARTINEZ G, PÉREZ LEIRÓS C, GRASSO E, RAMHORST R

Mar del Plata

Congreso; Reunión Anual Conjunta SAI-SAIC-SAFIS; 2018

SAI-SAIC-SAFIS

During the decidualization process the endometrial stromal cells modulate their secretome and display the ability to secrete many immuneregulatory and implantatory factors. Based in this ability, decidualized cell sense embryo quality allowing the implantation of normal development (ND) blastocysts while limit those with impaired development (ID). We propose that decidualized cells in response to ND blastocyst-derived factors promote a tolerogenic microenviroment while from ID-blastocyst sustain an hostile microenvironmet triggering the menstruation. Here we used and in vitro model of decidualization based on the Human endometrial stromal cell line (HESC) treated with medroxiprogesterone+dbcAMP in absence/presence of Blastocyst Conditioned Media (BCM) for 24h. BCM were recovered from 5 days blastocyst cultured individually, obtained from women with IVF or ICSI indication according to male evaluation, and classified as ND or ID blastocyst according to clinical standards. We observed that ND-BCM increased the frequency of decidualized cells producing the inmunomodulator peptide VIP evaluated by FACS, while ID-BCM decreased expression of its receptor VPAC1 relative to decidualized cells not treated with BCM (p