Ceramide 1 Phosphate Protects Human Endothelial Colony Forming Cells from Apoptosis and Increase Their Angiogenic Responses

SCHATTNER M; ZUBIRY PR; MENA HA; NEGROTTO S

Dublin

Congreso; 64th Annual ISTH SSC meeting; 2018

International Society on Thrombosis and Haemostasis

Ceramide 1 phosphate (C1P) is a bioactive sphingolipid released from dying cells and therefore highly augmented in damaged tissues. It is a well-known chemoattractant of macrophages and hematopoietic stem cells and, it has been recently reported a C1P proangiogenic action on mature endothelial cells. Considering the key role of human endothelial colony forming cells (ECFC) on vasculogenesis and tissue regeneration and that the effect of C1P in these processes remains unknown, we aimed to analyze the effect of C1P on the survival and angiogenic activity of ECFC. Human cord blood-derived CD34+ cells were cultured in EGM2 and, after 14-18 days, ECFC colonies were obtained. ECFC were treated with short- and long-chain C1P analogs like C8-C1P and C16-C1P respectively. N=3-6, *p