Interleukin-10 production and DNA polymorphisms in patients with Hemolytic Uremic Syndrome

BRUBALLA, ANDREA; FIORENTINO, GABRIELA ALEJANDRA; DE BRASI CARLOS; GONZALO PINEDA,; SANTIAGO, ADRIANA PATRICIA; ROSSETTI, LILIANA; RAMOS MV; MONGELOS, MICAELA; EXENI RAMON; ABELLEYRO, MARTIN; PALERMO, MARINA S.

Mar del Plata, Prov de Buenos Aires, Republica Argentina

Congreso; SAIC - SAI - SAFIS 2018; 2018

Title: Interleukin-10 production and DNA polymorphisms in patients with Hemolytic Uremic Syndrome.Authors: Micaela Mongelos1, Gonzalo Pineda1, Andrea Bruballa1, Ramón Exeni2, Adriana Santiago2, Gabriela Fiorentino2, Martín Abelleyro3, Liliana Rossetti3, Carlos De Brasi3, Marina Palermo1, María Victoria Ramos1.1Patogénesis E Inmunología De Procesos Infecciosos, IMEX. 2Hospital del Niño, San Justo, La Matanza. 3Genética Molecular de la Hemofilia, IMEX.Introduction: Development of Hemolytic Uremic Syndrome (HUS) is associated with Shiga toxin and inflammatory response. However, anti-inflammatory mechanisms could also be triggered to limit inflammation in vivo, such as production of the regulatory cytokine IL-10. Individual differences in IL-10 secretion associated with IL10-promoter single-nucleotide polymorphisms (SNP), such as rs1800896 (Legacy notation: -1082A>G) in which A- and G-alleles show low and high IL10 expression, respectively. Objective: The aim of this work was to evaluate the production of IL-10 and the specific SNP -1082A>G in patients with HUS.Materials and Methods: Blood samples were collected from HUS patients during the acute period and from Healthy Children (HC). Plasma and mononuclear cells (MNC) were isolated, and MNC were cultured with Medium or LPS (100ng/ml) for 20h. In parallel, absolute number of Monocytes (Mo) in MNC was calculated by flow cytometry. Protein expression of IL-10 was evaluated in plasma and MNC-supernatants by ELISA. DNA was obtained from MNC and IL10 SNP -1082A>G analysis was performed by allele specific-PCR (as-PCR) using a PCR-control template on CTLA4.Results: Plasmatic IL-10 concentration was increased in HUS patients (Mean±SEM (pg/mL): HUS=270.9±117.8*, HC=82.1±7.1, n=15, *p