IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MOUSE PATERNAL ALCOHOL EXPOSURE AFFECTS SPERMATOZOA AND COMPROMISES THE SURVIVAL OF ITS OFFSPRING
Autor/es:
CAMBIASSO MAITE; ALEJANDRA GISELLE ERLEJMAN; GABRIELA SALAMONE; STINSON MARCELO G; FONTANA VANINA ANDREA; GOTFRYD LUCILA; NAGUILA ZAIRA; CALVO JUAN CARLOS
Lugar:
MAR DEL PLATA
Reunión:
Congreso; LXIII REUNION ANUAL SAIC-SAI-SAFIS; 2018
Institución organizadora:
SAIC SAI SAFIS
Resumen:
Previously we observed that malealcohol consumption delayed embryo differentiation by deregulation ofperi-implantation events and alteration of embryo morphogenesis invitro.Aim:To evaluate the effect of paternal alcohol consumption on spermatozoa, embryoand litter survival on a mouse model. Methods:CF-1 male mice were exposed (Alcoholgroup, T) or not (Controlgroup, C) to 15% (v/v) ethanol in drinking water adlibitumfor 14days. Spermatozoa from cauda were obtained by swim-out to determine spermconcentration, motility, head decondensation(15, 30 and 60 min) and apoptosis by AnnexinV-FITC on both groups. Control and treated males were mated with non-treatedCF-1 females (1:1). Pregnant females (positive vaginal plug: day1) weresacrificed at day 2 of gestation to obtain 2-cell embryos which were cultured invitrofor 7 days and apoptosis was determined. Pregnancy outcomes were also evaluatedand litter mortality, weight and size registered. Results:Male alcohol consumption did not alter the number of mated female butsignificantly increased sperm head decondensationcompared to controls (p<0.05). There were no differences in spermconcentration and motility. Besides, we observed a higher number of oocytes atday 2 of gestation from females mated with treated males than control ones(p<0.01). Apoptosis was detected in peri-implantationembryos at 7 days of culture in vitrofrom treated vs. control groups. However, no differences were observed when weevaluated apoptosis on spermatozoa. After 21 days of pregnancy we registeredborn mice from both groups and observed during the first week of age anincreased number of deaths from treated group (p<0.001) compared to controlgroup. We observed less activity and poor fur in those pups from treated groupvs. control males. Conclusion:Short-term paternal alcohol consumption impairs sperm head decondensationaltering the embryo survival and pups behavior at an early age.