PATERNAL SHORT-TERM ALCOHOL CONSUMPTION ALTERS MOUSE EMBRYO-TROPHOBLAST DIFFERENTIATION DURING PERI-IMPLANTATION IN VITRO VIA PROGRAMMING EMBRYO DEATH.

GOTFRYD LUCILA; ERLEJMAN ALEJANDRA; FONTANA VANINA; FUENTES FEDERICO; CEBRAL ELISA; GABRIELA SALAMONE; CALVO JUAN CARLOS

Tokio

Congreso; International Federation of Placenta Associations (IFPA) 2018; 2018

IFPA

Aim: To evaluate the effect of paternal alcoholconsumption on peri-implantation embryo development, focusing on trophoblastand inner cell mass differentiation and apoptosis. Methods: CF-1 malemice were exposed (treated group, T) or not (control group, C) to 15% (v/v)ethanol in drinking water ad libitum for15 days, followed by mating with non-treated CF-1 females (1:1). Pregnant females(positive vaginal plug) were sacrificed at day 2 of gestation to obtain 2-cellembryos which were cultured in vitro for 7 days. Embryo compaction, blastocoelexpansion and hatching were assessed during days 1-3 of culture(preimplantation); embryo-trophoblast growth and differentiation were analyzedduring days 4-7 of culture (in vitro implantation).At culture day 7, fixed embryos (4% paraformaldehyde) were classified as type A(ICM: protruding aggregates of compact cells; TB (trophoblasts): symmetric monolayerof flat and elongated cells) or type B (ICM: disaggregated, few scattered or nocells; TB: asymmetric trophoblast outgrowth). Embryo apoptosis was determinedby annexin V-FITC and RT-PCR. Results: Male alcohol consumption for 15days delayed embryo differentiation by deregulation of peri-implantation eventsand alteration of embryo morphogenesis. Distribution of A and B type embryoswas significantly different (Fisher´s exact test) between C and T groups. ICM:53% A in C vs 10% A in T (p<0.001). TB: 60% A in C vs 26% A in T(p<0.01). After 7 days of culture, apoptotic cells were detected in embryos ofT vs C group. Conclusion: Short-term paternal alcohol consumptionimpairs mouse embryo peri-implantation by affecting ICM and TB differentiationand programming them to death. Considering that ICM participates in embryoformation and TB plays a relevant role in placental development, these effectsmight be crucial for further embryo survival.