SUPERANTIGEN INDUCE APOPTOSIS IN HUMAN NEOPLASTIC T CELLS.

MERCEDES PASTORINI; MERCEDES ALEMÁN; ALEJANDRA DUARTE; ALEJANDRO M. ROMÁN RISSO; MARINA SCARFO

Mar del Plata

Congreso; Reunión de las sociedades SAI -SAIC- SAFIS 2018; 2018

Superantigens (Sags) are bacterial and virus protein that share theability to activate large number of T-cells. Sags bind to major histocompatibilitycomplex (MHC) class II molecules as unprocessedproteins and interact with T cells expressing particular T-cell receptor(TCR) Vβ chains. We have previously shown that bacterialand mouse mammary tumor virus (MMTV)-encoded Sags inducedapoptosis of different murine-cognate lymphoma T cells both in vitroand in vivo. Moreover, bacterial T Sags increased the survival oflymphoma-bearing mice. Furthermore, we have recently describedthat Sags were also able to induce apoptosis of the Jurkat-establishedhuman cell line from an acute T-carrier leukemia of the Vβ8region in TCR. Thus, we now aimed to evaluate the effect of Sags onxenografts of Jurkat Cells in mice. For this propose we subcutaneouslyinoculated Jurkat cells (5x106) on Scid/Nod mice and then wetreated them intraperitoneally with specific Sag SEE (50µg). Afterward,we analyzed the tumor growth and Vb8+ cells in blood weeklyfor 6 weeks when final weight and histology were evaluated. Results:While SEE treatment did not affect immunological parametersor general fitness of mice, the tumor growth as well as lymphocytenumber in blood, were significantly reduced at week 5 and 4 respectively(p