IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Tear film hyperosmolarity disrupts ocular mucosal tolerance in a NF-kb-dependent fashion in dry eye disease
Autor/es:
GUZMAN M; SHIROMIZU, CAROLINA MAIUMI; GIORDANO, M; MIGLIO RODRIGUEZ M; FUENTES F; TREVANI, AS; KEITELMAN I; SABBIONE F; GALLETTI J
Lugar:
Mar del Plata
Reunión:
Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología. Mar del Plata 14-17 de noviembre, 2018, Argentina; 2018
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Introduction: Tear film hyperosmolarity resulting from increasedevaporation is commonly observed in dry eye and is known to haveproinflammatory effects on the ocular surface. Dry eye progressioninvolves disruption of ocular mucosal tolerance, but the events thatlead to it are unclear.Objective: To evaluate the effect of hyperosmolar stress on ocularmucosal tolerance.Methods: 8- to 12-week-old female Balb/c mice were instilled isoosmolar (0.3 Osm) or hyperosmolar (3 Osm) saline on both eyes 3times daily for 5 days. Ovalbumin (OVA) was instilled on both eyesat different time points. After subcutaneous immunization with OVAin adjuvant (day 8), induced T cell responses were measured by thedelayed-type hypersensitivity (DTH) response in the footpad (day15). In some experiments, T cells in draining lymph nodes (day 5)were evaluated by flow cytometry. In other experiments, mice wereexposed to hyperosmolar stress for 72h and then epithelial NF-kBactivation levels were analyzed by confocal microscopy in conjunctival whole-mounts (ImageJ software).Results: Compared to non-instilled immunized mice, OVA-instilledmice developed reduced DTH responses, as did their OVA+isoosmolar saline-instilled cage mates (n:6; p