IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Brucella abortus RNA contributes, along with its lipoproteins, to the down-regulation of IFN-γ-induced Major Histocompatibility Complex (MHC) Class II molecules expression on human monocytes, evading the host T cell surveillance
Autor/es:
TROTTA, ALDANA; GIAMBARTOLOMEI, GUILLERMO H.; SERAFINO, AGUSTINA; BARRIONUEVO, PAULA; MILILLO, M. AYELÉN; BALBOA, LUCIANA
Lugar:
Mar del Plata
Reunión:
Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVI Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); 2018
Institución organizadora:
SAIC SAI SAFIS
Resumen:
In order to persist inside the host, B. abortus must trigger different strategies to evade the adaptive T cell response. Previously, we demonstrated that B. abortus inhibits the IFN-γ-induced MHC-II surface expression on human monocytes. Consequently, the infected macrophages show a diminished antigen presentation to TCD4+ lymphocytes. B. abortus outer membrane lipoproteins ?bacterial structural components- are involved in MHC-II down-modulation through IL-6 secretion. Nevertheless, this phenomenon was less marked than that observed in the infection. This led us to think that there should have been another component associated to live bacteria implicated in MHC-II down-modulation. Actually, we have recently showed that B. abortus RNA, a PAMP associated to bacterial viability (vita-PAMP), is responsible for MHC-I down-modulation. Thus, the aim of this study was to analyse whether B. abortus RNA could also contribute to MHC-II down-modulation. For this, human monocytic THP-1 cells were incubated with B. abortus RNA in the presence of IFN-γ for 48 h. MHC-II expression (HLA-DR) was evaluated by flow cytometry. B. abortus RNA significantly (p