Dendritic cells maduration in spontaneous tumors

DUARTE, ALEJANDRA; CHIARELLA, PAULA; MONTAGNA, DANIELA R.; RUGGIERO, RAÚL A.

Ciudad Autónoma de Buenos Aires

Jornada; Frontiers in Bioscience 3; 2018

Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA) - CONICET - Partner Institute of the Max Planck Society)

Dendritic Cells (DCs) maturation is a key checkpoint in the initiation of T-dependent anti-tumor immunity. Most murine tumors of spontaneous origin display no evidence of immunogenicity, a feature putatively shared with many human tumors. To test the relationship between the immunogenicity of a tumor and its ability to promote the maturation of DC, we have used two murine tumors displaying widely different degrees of immunogenicity: the strongly immunogenic methylcholanthrene-induced MC-C fibrosarcoma and the spontaneous LMM3 carcinoma displaying undetectable immunogenicity. Bone marrow derived DCs were incubated with LMM3 or MC-C tumor lysates for 24 h. Then, markers of DC maturation as well as production of the inflammatory cytokines by DC were evaluated. DCs incubated with MC-C lysate displayed a significant increase of CD86 and MHCII markers of DC maturation and induced secretion of IL-12p70 and TNF-α as compared with control values observed using un-stimulated DC (p