IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Different regulation of circulating and renal cytokines in Shiga toxin treated IL10 deficient mice
Autor/es:
GONZALO PINEDA; M. FLORENCIA TODERO; ELSA ZOTTA; MARÍA VICTORIA RAMOS; ANDREA BRUBALLA; BÁRBARA REARTE; CATALINA ALBA-SOTO; MICAELA MONGELOS; MARTIN ISTURIZ; MARINA S. PALERMO
Lugar:
CABA
Reunión:
Simposio; Frontiers in Bioscience 3; 2018
Institución organizadora:
IBIOBA-MPSP
Resumen:
Hemolytic Uremic Syndrome is a disease triggered by Shiga toxin (Stx) characterized by hemolytic anemia, thrombocytopenia and renal failure. Although the role of inflammatory factors in this disease is well documented, the role of anti-inflammatory cytokines, such as IL-10, is less clear. Previously we demonstrated that IL-10 deficient mice (IL-10-/-) showed a higher survival after Stx2 inoculation, associated with lower renal damage reflected by reduced plasma creatinine levels. In this work, we studied the profile of pro- and anti-inflammatory factors in blood and kidney. Before or 3h after administration of 1LD100 of Stx2 e.v., IL-10-/- and Wt mice were euthanized and plasmas were collected. The kidneys were excised and the tissues homogenized in HEPES buffer. The plasma (pg/ml) and renal levels (pg/mg total protein) of IL-10, Corticosterone, IL-6 and TNF-α were quantified by ELISA.Even though IL-10 was not detected in Wt plasma, before and after Stx2, renal level of this cytokine was higher before than after Stx2 inoculation (Wt0h:57.31±8.16/WT3h:26.72±4.47*,*