IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of alpha-glucans from Mycobacterium tuberculosisin dendritic cell maturation and phagocytosis
Autor/es:
DUARTE ALEJANDRA; ALEMÁN MERCEDES
Lugar:
Aachen
Reunión:
Simposio; 15th International Symposium on Dendritic Cells; 2018
Resumen:
Tuberculosis remains the single largest infectiousdisease with two million deaths estimated to occur yearly. The spread of Mycobacterium tuberculosis (Mtb) from the lungs to other sites occur beforethe development of adaptive immune responses.The major carbohydrateconstituents from Mtb surface are α-glucans, a sugar which has been implicated in fungal virulence, and representup to 80% of the extracellular polysaccharides in Mycobacteria. Previous results demonstrated that reactive oxygen species (ROS) in neutrophils are generated by non-opsonized mycobacteria through α-glucansand that the lack of α-glucans in Mtb leads to the loss of their ability to induce ROS and also dendritic cell (DC) maturation and antigen presentation. Here we evaluated the role of α-glucans in the entrance of bacteria in DC and the endogenousproduction of ROS as effectors of the maturation of DC.Results: Monocyte-derived DC were incubated 24 hours with LPS and thereafter were treated with Mtb (H37Rv) subjected or not to a-amyloglucosidasetreatment (Mtb-e) for 15 min with or without H2O2. Thereafter maturation markers were evaluated by cytometry. Expression of CD86 (p