POTENTIAL OF MALDI-TOF AS A DIAGNOSTIC TOOL IN SEPSIS

MAURICIO SANTOS; MÓNICA PRIETO; MARTÍN A. ISTURIZ; MARÍA FLORENCIA TODERO; ROXANA PAUL; NORBERTO SÍMBOLI; BÁRBARA REARTE; JOHANA MONTESERIN; BEATRIZ LÓPEZ; NOEMÍ YOKOBORI

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI SAFIS 2018; 2018

Sepsis has a bi-phasic nature with a hyper-inflammatory phase followed by an anti-inflammatory response. Globally, it is one of the main causes of death in ICUs. However, clinically, is difficult to define a therapeutic approach due to the lack of tools to determine the phase that the patient is going through. Our aim was to investigate the usefulness of plasma ?fingerprints? using MADI-TOF MS technology as rapid instrument for determination of pro-inflammatory or anti-inflammatory states in sepsis. For this, LPS-challenged murine models emulating the different phases of sepsis (proinflammatory (PRT) and anti-inflammatory (ANT) phases) versus untreated controls (Ctrl) were evaluated. To better characterize the different phases, in parallel, cytokines profiles, hematologic, metabolic and immunologic parameters were determined in plasma. Peripheral blood leukocytes count was decreased in PRT whereas in the ANT phase was increased mainly associated with an increase in neutrophils (Leukocytes (106/ml): Ctrl= 6.6±0.5; PRT= 1.9±0.2*; ANT= 11.2 ±0.7&; *&p