Decreased angiogenic activity of coxsackievirus B3-infected endothelial colony forming cells

MENA, HA; GÓMEZ, R; ETULAIN, J; NEGROTTO, S; ZURIBY, PR; SCHATTNER, M

Congreso; SAIC-SAFIS 2018; 2018

Coxsackievirus B3 (CVB3) is a globally prevalent Enterovirus frequently associated with viral myocarditis that may lead to dilated cardiomyopathy and heart failure. Local or homed progenitor cells are involved in myocardial repair, especially endothelial progenitor cells which have a lead role in new blood vessel formation and tissue revascularization. We have previously demonstrated that CVB3 infects and replicates in ECFC and although it failed to affect ECFC survival, it significantly reduced proliferation and tubulogenesis. Here, we aimed to study the effect of CVB3 on other functional responses of late outgrowth endothelial colony forming cells (ECFC) involved in new vessel formation.Cord blood-derived CD34+ cells were seeded in EGM2 and ECFC colonies were obtained after 14-21 days. ECFC were incubated with CVB3 at a multiplicity of infection (MOI) 0.1 or 1 for 1 hour and then washed to remove the remaining virus. N=3-5. *p