IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF IL-10 ON RECRUITMENT OF PMN-LIKE MYELOID CELLS (PLMCs) DURING THE ESTABLISHMENT OF BACTERIAL ENDOTOXIN-INDUCED TOLERANCE
Autor/es:
GONZALO PINEDA ; BÁRBARA REARTE; MARLINA OLYISSA CORDOBA MORENO; VICTORIA RAMOS; ADRIANA FONTANALS; MARTIN AMADEO ISTURIZ
Lugar:
Ciudad de Buenos Aires
Reunión:
Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS 2017; 2017
Resumen:
In sepsis by Gram-negative, endotoxin -LPS- is capable of inducing a tolerance state to LPS (TOL), the initial step of immunosuppression, the main cause of death. We have previously demonstrated that the TOL in absence of IL10 could improve innate immune response mainly mediated by PMN recruitment. The aim of this work was to evaluate the IL-10 effect on the recruitment of PLMCs with high levels of CD11b+/GR1+ and CD11b+/Ly6G+ in TOL. For this, BALB/c wild type(WT) and IL-10 deficient(KO) mice are used. The TOL establishment is achieved with a regimen of increasing doses of LPS (0.25-10ug/mouse) for 10 days. Glucocorticoids (GC) levels are measured by RIA. Flow cytometry was applied to measure PLMCs. During the establishment of TOL an increase in the percentage of recruitment of PLMCs is shown, this increase is greater in KO: %cells-spleen: WT-TOL:10.99±1.24, KO-TOL:32.26±4.93*, *p