CONTRIBUTION OF MESENCHYMAL STEM CELLS TO ENDOTHELIAL REPAIR IN HEMOLITIC UREMIC SYNDROME

MARTIRE GRECO, DAIANA; FUMENTO,VA; LANDONI, VERONICA I.; NORRIS, A; RODRIGUEZ RODRIGUES, N; FERNANDEZ, GABRIELA C.; LUZZANI, C; CASTILLO, LUIS A.; MIRIUKA, S

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017

Mesenchymal stem cells (MSC) are multipotent cells that possessknown tissue regenerative properties. Induced Pluripotent StemCell-Derived Mesenchymal Stem Cell (iPS-MSC) has similar characteristicsas MSC. Endothelial tissue damage in the renal glomerulusis one of the most relevant issues that cause kidney failure inHemolytic Uremic Syndrome (HUS). Our aim was to investigate ifiPS-MSC were able to contribute in the repair of endothelial damagecause by the exposure to lipopolysaccharide (LPS) and Shiga toxin2 (Stx), the two most important etiological mediators in HUS. For thispurpose, we used an in vitro model of endothelial damage using thecell line HMEC-1 incubated with LPS and/or Stx for 24 h. We foundthat iPS-MSC exposed to LPS decreased their migratory capacitymeasured as the migrated area after a wound in the cell monolayercompared to Control cells, but the combination of LPS+Stx reversedthis effect (area cm2 (x102), Control:5,10±0,04; LPS:3,60±0,02*;Stx: 3,80±0,03*;LPS+Stx:6,50±0,04#,*vs. Control and #vs. LPSor Stx, p