IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PLATELETS DIRECTLY INTERACT WITH Brucella abortus AND MODULATE INFECTED HUMAN MONOCYTES TOWARDS A PRO-INFLAMMATORY PROFILE
Autor/es:
M. VICTORIA DELPINO; GUILLERMO H. GIAMBARTOLOMEI; M. AYELÉN MILILLO; ANA M. RODRÍGUEZ; PAULA BARRIONUEVO; ALDANA TROTTA; LIS N. VELÁSQUEZ; ROBERTO G. POZNER
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Biociencias 2017.; 2017
Resumen:
Brucellosis is an infectious disease elicited by bacteria of the genusBrucella. Platelets have been described as mediators of hemostasis.However, they have recently got involved in the modulationof innate and adaptive immune responses. We have previouslyreported that platelets modulate B. abortus-mediated infection ofhuman monocytes. The first aim of this study was to characterizethe interaction between platelets and B. abortus. For this, humanplatelets were co-incubated with B. abortus-GFP for 4 h and the interactionwas quantified by confocal microscopy and flow cytometry.Our results showed that platelets are able to interact with bacteriain a dose-dependent fashion. Moreover, this interaction stimulatedplatelet activation, measured as fibrinogen binding and P-selectinexpression (p