IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Prokaryotic RNA modulates endothelial-neutrophil interactions
Autor/es:
RODRIGUEZ RODRIGUES, N; BIRNBERG-WEISS, FEDERICO; MARTIRE GRECO, DAIANA; FERNANDEZ, GABRIELA C.; CASTILLO, LUIS A.; LANDONI, VERÓNICA I.
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017
Resumen:
Human neutrophils (PMN) are able to sense bacterial viability, activatingand generating a response only against live bacterial stimuli.In this regard, we have demonstrated that prokaryotic RNA (pRNA),present only in live bacteria, is capable of triggering PMN activationand the generation of bactericidal responses. Because endothelialcells can actively regulate the migration of PMN through the endotheliumby the expression of adhesion molecules and chemokines,our objective was to determine if pRNA can directly activatethe endothelium and modulate the interaction with PMN. In all experiments,a microvascular endothelial cell line (HMEC-1) was stimulatedwith RNA (1 μg/ml) extracted from E. coli (pRNA) or from humaneukaryotic (mononuclear) cells (eRNA), or left untreated, for 24hs. After stimulation, the expression of ICAM-1 (CD54) in HMEC-1,measured as the MFI by FACS was increased in pRNA-treated cellscompared to the other groups (p