Acidic preconditioning improves endothelial progenitor cell survival and angiogenesis under proinflammatory conditions and post-ischemic tissue regeneration in a murine model of type II diabetes

NEGROTTO S; SCHATTNER M; BOISSON-VIDAL C; MENA HA

Berlin

Congreso; ISTH Congress; 2017

ISTH

We have previously demonstrated that acidic preconditioning exacerbates angiogenic responses of human late outgrowth endothelial colony forming cells (ECFC). We now aimed to analyze whether this strategy also improves ECFC survival and functionality in the presence of damage associated molecular patterns (DAMPs) highly augmented in ischemic milieu or in diabetes, where tissue regeneration is compromised. Cord blood-derived ECFC in EGM2 were exposed at pH 6.6 for 6 h (preconditioned) or pH 7.4 (control) and then medium was replaced by fresh EGM2 at pH 7.4. N=4-6, *p