IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ROLE OF ALPHA-GLUCANS FROM Mycobacterium tuberculosis IN DENDRITIC CELL MATURATION AND PHAGOCYTOSIS
Autor/es:
ALEMÁN MERCEDES; DANIELA MONTAGNA; ALEJANDRA DUARTE
Lugar:
buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Tuberculosis remains the single largest infectious disease with twomillion deaths estimated to occur yearly. The spread of Mycobacteriumtuberculosis (Mtb) from the lungs to other sites occur beforethe development of adaptive immune responses. The major carbohydrateconstituents from Mtb surface are α-glucans, a sugar whichhas been implicated in fungal virulence, and represent up to 80% ofthe extracellular polysaccharides in Mycobacteria. Previous resultsdemonstrated that reactive oxygen species (ROS) in neutrophils aregenerated by non-opsonized mycobacteria through α-glucans andthat the lack of a-glucans in Mtb leads to the loss of their ability to induceROS and also dendritic cell (DC) maturation and antigen presentation.Here we evaluated the role of α-glucans in the entranceof bacteria in DC and the endogenous production of ROS as effectorsof the maturation of DC. Results Monocyte-derived DC wereincubated 24 hours with LPS and thereafter were treated with Mtb(H37Rv) subjected or not to a-amyloglucosidase treatment (Mtb-e)for 15 min with or without H2O2. Thereafter maturation markerswere evaluated by cytometry. Expression of CD86 (p