IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE TUBERCULOUS PLEURAL EFFUSION ALTERS THE METABOLIC REPROGRAMMING OF M1 ACTIVATED MACROPHAGES
Autor/es:
GENOULA, MELANIE; MORAÑA, EDUARDO; SCHIERLOH, PABLO; MARÍN FRANCO, JOSÉ LUIS; FERREYRA, MALENA; SASIAIN, MARIA DEL CARMEN; DUETTE, GABRIEL; PALMERO, DOMINGO; BALBOA, LUCIANA
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017
Resumen:
In response to infection, activation of the host immune cells is accompanied by a switch in the bioenergetic pathway from oxidative phosphorylation to aerobic glycolysis, which is requires for the production of antimicrobial and pro-inflammatory effector molecules. Particularly the pro-inflammatory macrophages or M1 undergo this metabolic reprogramming governed by HIF-1α. While M1 are key players to combat intracellular pathogens such as Mycobacterium tuberculosis (Mtb), the bacilli display several strategies to counterattack. Since this metabolic reprogramming is associated with M1 functions, we hypothesize that Mtb may perturb it to facilitate its persistence. Then we wondered whether Mtb infection can alter the metabolic reprogramming of M1. To test it, we polarized human macrophages towards M1 with IFN-γ/LPS and treated them with the acellular fraction of tuberculous pleural effusions (PE) mimicking those soluble factors released locally during the infection. Glucose and lactate were determined by colorimetric methods; HIF-1α, Glut-1 and glucose uptake by FACS; IL-1β and TNF-α by ELISA; and bacterial loads by colony forming units. The treatment with PE increased the expression of the glucose transporter Glut-1 (p