RELEVANCE OF THE BLASTOCYST CONDITIONED MEDIA ON IMMUNOTOLERANCE: FOCUS ON THE INFLAMMATORY RESPONSE

CLAUDIO RUHLMANN; MARÍA SOLEDAD GORI; ELIZABETH SOCZEWSKI; ROSANNA RAMHORST; MARCELA IRIGOYEN; LUCILA GALLINO; ESTEBAN GRASSO; CLAUDIA PÉREZ LEIRÓS; GUSTAVO MARTÍNEZ; DANIEL PAPARINI; LAURA FERNÁNDEZ

Buenos Aires

Jornada; Jornadas Interdisciplinarias de Química Biológica; 2017

Departamento de Química Biológica, FCEN-UBA

Implantation is associated with a sterile inflammatory response that should be later controlled to a tolerogenic microenvironment by maternal and blastocyst-derived factors. During decidualization, cells undergo reticular stress and unfolded protein response, allowing them to expand their endoplasmic reticulum and change their secretome increasing immunomodulators? production. We investigated whether human Blastocyst Conditioned Media (BCM) could control the initial inflammatory response during the implantation period.Human endometrial stromal cell line (HESC) were decidualized or not with medroxiprogesterone + dbcAMP. Then, HESC cells were treated with human BCM for 24 h. Gene and protein expression was evaluated by real time PCR or FACS, respectively. An in vitro implantation model based on co-culture of blastocyst-like spheroids (BLS) from Swan-71 trophoblast cells line over decidualized-HESC cells and a transwell migration system for Tregs recruitment were used to evaluate decidual functionality. We observed increased expression of interleukin-8 (CXCL8) (1.21±0.06), stromal derived factor 1 (CXCL12) (1.43±0.18), and IL-1b intracellular protein production (4.72±1.21) after the decidualization (P