IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Euglobulin Lysis Time (ELT) Behaviour in Patients With Abnormal Clot Solubility Test (CST)
Autor/es:
BLANCO A; ROMERO ML; INGRATTI M; SÁNCHEZ LUCEROS A; VERA MORANDINI M; REMOTTI L; AGAZZONI M; GROSSO S
Lugar:
Dublin
Reunión:
Congreso; 64th Annual Scientific and Standardization Committee (SSC); 2018
Institución organizadora:
International Society on Thrombosis and Haemostasis, Scientific and Standardization Committee (SSC)
Resumen:
Background: FXIII stabilizes fibrin and protects it from fibrinolysis;Alpha 2-antiplasmin (a2PI) is involved. Independently of a2PI, FXIII plays a role in fibrin stability increasing its resistance to fibrinolysis.Aims: The ELT behaviour was analysed, in patients with bleeding and abnormal CST, in order to see if any relationship could exist between both tests, considering that CST depends on FXIII and ELT reflects fibrinolysis in absence of a2PI.Methods: Subjects: 180 patients with bleeding symptoms. CST and ELT were tested, in addition to other platelet and coagulation studies. A retrospective analysis was performed. CST: samples were clotted by CaCl2 (25mM); after 30min at 37°C, acetic acid (2%) was added (RV> 72h). ELT: euglobulin fraction (a2PI free) wassuspended in borate solution (0.9%) and clotted by CaCl2 (25mM) at 37°C(RV: 90-240min).Results: Patients were classified according to CST results as either normal (111/180; 61,7%) or abnormal (69/180; 38.3%). As displayed in the table only 4 presented abnormal ELT in the group of patients with normal CST; 11 patients showed abnormal ELT in the group with abnormal CST. Borderline ELT values were found in 4 and 1 patients with normal and abnormal CST respectively. The frequency of ELT abnormalities among patients with abnormal CST was higher (chisquare test P=0.011) than among those with normal CST. Other abnormalities,like platelet alterations, coagulation factors disorders or both, were detected in either patients with normal (96/111; 86.5%) or abnormal (55/69; 79.7%) CST; the differences were not significant (chi square test P=0.23).Conclusions: The study showed that the number of patients with abnormal ELT was significant higher among those with abnormal CST, suggesting a possible relationship between FXIII activity and fibrinolytic function, independently of a2PI. Further studies could help to elucidate the role of direct modulation of fibrin structure by FXIII, compared with a2PI cross-linking.