ANALYSIS OF MYC REARRANGEMENTS IN PATIENTS WITH MULTIPLE MYELOMA

GUASCH LG; STELLA F.; GONZÁLEZ M,; PEDRAZZINI E.; SLAVUTSKY I

Buenos Aires

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica

Multiple myeloma (MM) is a malignant B-cell lymphoproliferative diseasecharacterized by the infiltration of clonal plasma cells in the bone marrow. At the genetic level, it is a heterogeneous disorder characterized by multistage accumulation of genetic abnormalities deregulating different pathways.The MYC proto-oncogene is a transcription factor that plays a role in cell cycle progression, apoptosis and cellular transformation. Rearrangements of this gene have been thought to be rare events in MM. In this study, we have evaluated MYC rearrangements in MM patients by FISH (fluorescence in situ hybridization) analysis. A total of 46 MM patients (26 females; mean age: 59.5 years; range: 46-86 years) were analyzed. Cytogeneticstudy was performed on unstimulated bone marrow cultures. FISH analysis was performed using TP53, RB1, IGH and MYC probes(Live-Lexel, Argentina) according to manufacturer´s protocol.MYC rearrangements were observed in 25 (54.3%) patients: 19 (76%) cases showed split signals, 5 (20%) had MYC gainsand one case (4%) showed both split and gain. The correlation with recurrent alterations detected by FISH revealed a significant association with the presence of IGH rearrangements (p