HISTAMINE RECEPTORS H3/H4 INVOLVEMENT IN A MOUSE MODEL OF DERMATITIS.v

SOLEDAD GORI; GABRIELA SALAMONE; JULIETA ALCAIN; MÓNICA VERMEULEN

Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2016

SAI

Dermatitis is an inflammatory disease characterized by animbalance of the immune response toward a Th2 profile, whosemain inflammatory mediator is histamine (HIS). HIS exerts itsactions through four receptors (H1-H4). Previously, we demonstrated,using a model of pulmonary inflammation to OVA, that HISis able to induce the recruitment of CD8+ T lymphocytes in thelung of mice. The purpose of the present work was to study themodulatory role of HIS in another inflammatory disease affectingthe skin, contact dermatitis, and the role of H3 and H4 receptors.For this, we developed a mouse model of dermatitis using theirritating agent dinitrophenyl benzene (0.7% DNFB in acetone/water). For this, 20 ìl of DNFB (or vehicle) was administered for4 consecutive days on both ears, along with 20 ìl of a H3/H4receptor antagonist (thioperamide, Thio, 10-7M) on the right ear.Seven days after the first administration, DNFB (0.2% in PBS)was administered intraperitoneally in all mice. Topical treatmentof the injured zone with Thio partially reversed the symptomsof the disease associated with inflammation in the applicationarea (Ct, 0.267 mm ± 0.033 mm; DNFB, 0.667 mm ± 0.044 mm;DNFB+Thio, 0.416 mm ± 0.072 mm. p