IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NO NEURONAL CHOLINERGIC SYSTEM INFLUENCE IN THE PROGRESSION OF U251MG HUMAN GLIOBLASTOMA CELL LINE AND ITS IMPACT ON THE IMMUNE RESPONSE
Autor/es:
SOLEDAD GORI; JULIETA ALCAIN; MARIANELA CANDOLFI; MARIELA MORENO; WALTER SCORDO; GABRIELA SALAMONE; LUCIANA MOVERER; ANTONELA ASAD; MÓNICA VERMEULEN
Lugar:
Mar del Plata
Reunión:
Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2016
Institución organizadora:
SAI
Resumen:
Glioblastoma multiforme (GBM) is the deadliest and most commontype of human primary brain tumor. This tumor is defined bythe hallmark features of uncontrolled cellular proliferation, diffuseinfiltration, propensity for necrosis, robust angiogenesis, intenseresistance to apoptosis and genomic instability. Acetylcholine(ACh) is a neurotransmitter which can also modulates cell survival,proliferation and differentiation in neuronal and non-neuronal cellssuch as immune cells, which has been referred to as a ?nonneuronalcholinergic system?. The aim of this work is to elucidatethe relevance of the non-neuronal cholinergic system in the interactionimmune-GBM cells. Methods: Human U251 GBM cells werecocultured with human dendritic cells (DC) obtained from healthyadult volunteer. Mononuclear cells were isolated from buffy coatsof healthy adult nonsmoker volunteer and CD14+ cells were thenisolated by positive selection and then were cultured with GM-CSFand IL-4. The cells were cultered in the presence of cholinergic agonists(carbachol, muscarine and nicotine) and antagonists (atropineand mecamelamine) and evaluated DC maturation. Results: Wefound that coculture of dendritic cells with U251 cells in the presenceof carbachol and nicotine treatment increased the release ofIL-8 (P