IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MODULATION OF GAMMA/DELTA T CELL ACTIVATION BY NEUTROPHIL ELASTASE
Autor/es:
KEITELMAN, IRENE; JANCIC, CAROLINA; TOWSTYKA, NADIA; GEFFNER, JORGE; SABBIONE, FLORENCIA; TREVANI, ANALIA
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión anual de la Sociedad Argentina de Inmunología; 2016
Resumen:
)amma/delta T cells play a critical role in the recruitment andactivation of neutrophils at the sites of infection. +n addition, neutrophilscan regulate gamma/delta T cell activity. 9e have previouslyreported that elastase secreted by neutrophils enhances gamma/delta T cell activation triggered by antiC& antibodies. 0oY, Yeaim to investigate the mechanism by Yhich elastase modulatesgamma/delta T cell activation. )amma/delta T cells Yere purifiedfrom human peripheral blood mononuclear cells, by using anantiTC4 gamma/delta /icro$ead isolation Mit. 0eutrophils Yereisolated by dextran sedimentation. After purification, gamma/deltaT cells Yere stimulated Yith antiC& antibodies ng/ml, andcultured Yith or Yithout neutrophils. After h, Ye analyzed ingamma/delta T cells, the C& expression by floY cytometry andthe +F0gamma and T0Falpha production by EL+SA. 9e evaluatedcell conjugates formation and elastase expression by confocalmicroscopy. Finally, Ye analyzed the expression of the proteasesactivated receptor 2A4 by floY cytometry and the functionalityof 2A4 by using the agonist thrombin 7/ml, and theantagonist 49, u/. )amma/delta T cells and neutrophilsformed cell conjugates, v of these conjugates recruitedelastase at the celltocell contact p., n. )amma/deltaT cells expressed 2A4 on cell surface p., n. PAR1Yas activated by thrombin treatment, measured as an increasedin C& expression p., n and +F0gamma p., nand T0Falpha p., n production. /oreover, in presenceof 2A4 antagonist, 49,, neutrophils did not potentiate theactivation of gamma/delta T cells induced by antiC& antibodies,measured through the expression of C& p., n and theproduction of +F0gamma p., n and T0Falpha p.,n. 1ur results indicate that 2A4 mediates the effect of neutrophilselastase on gamma/delta T cells activated through C&.