EFFECTS OF SHORT-TERM FEVER-RANGE HYPERTHERMIA ON NEUTROPHIL RESPONSES TO PSEUDOMONAS AERUGINOSA.

KEITELMAN, IRENE; TOWSTYKA, NADIA; JANCIC, CAROLINA; SABBIONE, FLORENCIA; GUZMÁN, MAURICIO; GÓMEZ, MARISA; GAI, CONSTANZA; GALLETTI, JEREMIAS G.; TREVANI, ANALIA

Mar del Plata

Congreso; Reunión anual de la Sociedad Argentina de Inmunología; 2016

Fever response is a hallmarM of infection and inflammatorydisease, YorMing as an alert system that promotes immune surveillanceduring challenge by invading pathogens. 2reviously Yedetermined that h of hyperthermic conditions .uC significantlyincreased 0ETosis induced by 2/A. HoYever, cytoMine releasein response to L2S Yas significantly inhibited. +n this study, Yeaim to determine if short term hyperthermia is able to modulate0ET release and other neutrophil 2/0 functions in response tothe opportunist pathogen 2seudomonas aeruginosa 2a. Human2/0 Yere incubated Yith or Yithout 2a 2A1 strain /1+ at uC or .uC for h, and then cultured for different periodsat uC according to the evaluated function. The secretion of +LYas determined by EL+SA and elastase secretion Yas assayedby degradation of a specific substrate. 0ETosis Yas evaluatedby determining colocalization of elastase and &0A by confocalmicroscopy C/, and &0A released by fluorometry. Autophagylevels Yere determined by quantification of vesicular LC$/cellby C/. 4esults indicated that h of exposure to .uC and additionalculture at uC did not modulate the capacity of bacteriato induce &0A release at , , or h n. Hyperthermia didnot affect 0ET release induced by 2a evaluated by C/ at , and h postthermic treatment n. HoYever, elastase levelsdetected in 2astimulated2/0 supernatants Yere reduced incells exposed to hyperthermia n p. suggesting that partof it could have been released not only by 0ETosis but also duringbacterial phagocytosis. Similar findings Were obtained When autophagy levels Yere evaluated n. As it Yas observed usingL2S as stimulus, hyperthermia inhibited +L secretion induced by2a evaluated at , , or h poststimulation n. +n summary,these results suggest that shortterm hyperthermia differentiallyaffects not only 0ETosis in response to 2/A and 2a, but alsodistinct neutrophils responses induced by 2a