CHARACTERIZATION OF URATE MONOSODIUM CRYSTALS EFFECT ON GAMMA/DELTA T CELLS

TOWSTYKA, NADIA; GEFFNER, JORGE; SABBIONE, FLORENCIA; TREVANI, ANALIA; KEITELMAN, IRENE; JANCIC, CAROLINA

Mar del Plata

Congreso; Reunión anual de la Sociedad Argentina de Inmunología; 2016

The inflammatory effect of uric acid depends on its precipitationinto monosodium urate crystals /S7C. /S7C activate differentintracellular pathYays, i.e. SyM Minase and 2+-. )amma/delta Tcells act as sensors of cellular stress and infection. They recognizedanger and pathogen-associated molecular patters, such asphosphoantigens. 9e have previously reported that /S7C activategamma/delta T cells. 0oY, Ye aim to investigate Yhether /S7Cmodulate gamma/delta T cell activation triggered by phosphoantigens,and the mechanism involved in gamma/delta T cell activationby /S7C. )amma/delta T cells Yere purified from human peripheralblood by using an antiTC4 gamma/delta /icro$ead isolationMit. After purification, gamma/delta T cells Yere incubated or notYith /S7C ug/ml, h. Then, Ye analyzed C& expressionby floY cytometry, and +F0gamma and T0Falpha productionby EL+SA. To investigate the costimulatory effect of /S7C, Yetreated gamma/delta T cells Yith the phosphoantigen H/$22 u/ and then Ye incubated cells Yith /S7C. Finally, to study themechanism involved in gamma/delta T cell activation by /S7C,Ye treated cells Yith or Yithout a protein Minase SyM inhibitor,)S . u/ )amma/delta T cells costimulated Yith /S7Cand H/$22 produced higher levels of +F0gamma and T0Falphacompared to gamma/delta T cells activated by /S7C or H/$22alone p., n. 1n the other hand, treatment of gamma/delta T cells Yith SyM inhibitor )S, completely abrogated theincrease in C& expression induced by /S7C p., n. 1urresults indicate that gamma/delta T cells activated Yith H/are able to recognize /S7C efficiently, suggesting a possiblyrole in sensing cell death molecular patterns released during aninfection. Also, Ye shoY that the protein Minase SyM is involved ingamma/delta T cells activation by /S7C.