IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Analysis of basal chromosomal aberrations and micronucleus frequency in patients with chronic lymphocytic leukemia
Autor/es:
CARMEN STANGANELLI; MARCELA GOZÁLEZ CID; PATRICIA DOS SANTOS; MICAELA PALMITELLI; IRMA SLAVUTSKY
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Científica de la Sociedad Argentina de Investigación Clínica; 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Chronic lymphocytic leukemia (CLL) is a B-cell malignancy characterized by a highly heterogeneous clinical evolution. The mutational status of the immunoglobulin heavy-chain variable (IGHV) region permits to divide this pathology in two different subgroups: mutated (M) CLL with less than 98% homology with respect to the germline counterpart, associated to better prognosis, and unmutated (UM) CLL with ≥98% homology, related to a poor outcome. Chromosome aberrations (CA) and micronucleus (MN) techniques represent different forms to evaluate genomic instability; MN provides a measure of both chromosome breakage and chromosome loss. In this study we analyze the basal frequency of CA and MN in 22 CLL patients (13 males; mean age: 64.6 years), and 7 controls. Cytogenetic analysis was performed on stimulated peripheral blood lymphocyte cultures. For each patient, CA was evaluated on 50 metaphases meanwhile 250 interphase nuclei were analyzed for MN frequency. IGHV mutational status was determined by PCR and sequencing. Forteen (63.6%) CLL patients presented metaphases with chromatid breaks, gaps and dicentrics. Analysis of data showed increased MN frequency (2.87±0.38%) as well as the number of metaphases with CA (1.77±0.47) in CLL cases compared to controls (0.4±0.02% and 0.63±0.2, respectively) (p=0.01). When patients were analyzed according to the IGHV mutational status, M (10) and UM (12), no differences between groups in MN (3.17±2.18% and 2.52±0.98%) and CA (1.10±1.45 and 2.33±2.50, respectively) frequencies were observed. To our knowledge, this is the first study measuring MN and CA in CLL cases. Our results reflect the presence of genomic instability in this pathology and suggest that similar mechanisms may be present in M and UM subgroups of patients.