Altered neutrophil activation in absence of IL-10 in a murine model of hemolytic uremic syndrome.

PINEDA, GONZALO EZEQUIEL; BRUBALLA, ANDREA; ISTURIZ, MARTIN AMADEO; RAMOS MV; REARTE, BARBARA; FERNANDEZ-BRANDO, RJ; ALBA SOTO, CATALINA; CORDOBA, MARLINA; MEISS, R; PALERMO, MARINA SANDRA

Mar del Plata, Prov Buenos Aires, Argentina

Congreso; LXI Reunion Cientifica Anual de la Sociedad Argentina de Investigacion Clinica. LXIV Reunion Cientifica Annual de la Sociedad Argentina de Inmunologia; 2016

Altered neutrophil activation in absence of IL-10 in a murine model of hemolytic uremic syndrome.Gonzalo Ezequiel Pineda1, Barbara Rearte2, Marlina Córdoba Moreno2, Andrea Bruballa1, Romina Fernandez-Brando1, Roberto Meiss3, Martin Isturiz2, Catalina Alba-Soto4, Marina Palermo1, Maria Victoria Ramos1.1Laboratorio de Patogenesis e Inmunologia de Procesos Infecciosos, IMEX-Academia Nacional de Medicina.2Laboratorio de Fisiologia de los procesos Inflamatorios, IMEX-Academia Nacional de Medicina.3Departamento de Patologia, Instituto de Estudios Oncologicos, Academia Nacional de Medicina4Instituto de Investigaciones en Microbiología y Parasitología Médicas (IMPaM). Facultad de Medicina. Universidad de Buenos Aires.Hemolytic Uremic Syndrome (HUS), is a systemic disease caused by circulating Shiga-toxin (Stx) which mainly damages kidney. Besides Stx2, inflammatory response mediated by neutrophils (PMN) is essential to HUS evolution. We have demostrated that mice lacking IL-10 (IL-10-/-) had a higher survival rate to Stx2 than controls. The aim of the work was to determine the role of PMN in HUS evolution in absence of IL-10. Before and 24h, 48h and 72h after of 1LD100 of Stx2 e.v., IL-10-/- and control mice were sacrificed. Total leukocytes were counted and PMN were identified as Ly6G+CD11b+ cells in blood and bone marrow. PMN production of reactive oxygen species (ROS) was evaluated without stimulus (basal) or after PMA stimulation, with DHR by FACS. In basal condition both strains showed similar absolute number of PMN in blood, although the percentage of PMN in bone marrow was increased in IL-10-/- mice (%PMN:IL-10-/-= 52.8±4.1,control=39.9±1.9*,*p