Acidic preconditioning improves endothelial progenitor cell survival and functionality under inflammatory conditions

SCHATTNER M; MENA HA; NEGROTTO S; OJEDA LE

Montpellier

Congreso; Annual Meeting of the SSC ISTH Congress; 2016

ISTH

We have previously demonstrated that acidic preconditioning exacerbates angiogenic responses of late outgrowth endothelial progenitor cells (ECFC). In the present work, we aimed to analyze whether this strategy is also effective to improve ECFC functionality under stressful conditions typically observed in inflammation or cardiovascular diseases such as high glucose, monosodium urate (MSU) crystals, TNF or extracellular histones. Acidic preconditioning was achieved by incubating cord blood-derived ECFC in EGM2, pH 6.6 for 6 h (preconditioned) and then medium was replaced by fresh EGM2, pH 7.4. ECFC incubation at pH 7.4 for 6 h was conducted as control. Viability (nuclear morphology analysis by fluorescence microscopy), wound healing (scratch assay) and tubule formation (matrigel) were analyzed 24 h after treatment. Data are shown as % of control, n=3-4, *p