Effect of IL-10 on recruitment and function of neutrophil-like myeloid cells during the course of immunosuppression induced by bacterial endotoxins

CÓRDOBA MORENO M; FONTANALS A; PINEDA G; MEISS R; ISTURIZ MA; RAMOS MV; REARTE B.

Mar del Plata

Congreso; Reunión Anual de la Sociedad Argentina de iNMUNOLOGÍA; 2016

Sociedad Argentina de Inmunología

In sepsis caused by Gram negatives, lipopolysaccharides (LPS) are associated to the immunosuppression (IS), the main cause of death. Numerous agents such as the IL-10 have been proposed as immunosuppressant. We have shown that even in IL-10 deficient BALB/c mice (KO) it was possible to establish a humoral IS similar to the wild type (WT). The aim of this work was to evaluate the role of IL-10 on the innate immunity alterations observed in LPS induced IS, done through cytometric and morphologic studies. First we determined the leukocyte number in peritoneum and peripheral blood (PB) after IS. In PB the leukocyte numbers was increased mainly associated with an increase in neutrophils (PMN) numbers in ISKO mice (Leukocytes (106/ml): ISWT:4±0.7; ISKO:13.7 ±0.4*; *p