IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genetic diversity of Mycobacterium avium complex by MIRU-VNTR analysis
Autor/es:
ROMERO M; G TRAVERIA; R D MOYANO; M P SANTANGELO; M I ROMANO; B IMPERIALE; ALVARADO PINEDO MF; MORCILLO NS
Lugar:
Catania
Reunión:
Congreso; 37° Congreso Anual de la Sociedad Europea de Micobacteriología (ESM).; 2016
Resumen:
Introduction. Mycobacterium avium complex (MAC) are opportunistic pathogens causing mycobacteriosis in humans and animals. M. avium sp. avium (MAA), M. avium sp. hominissuis (MAH) and M. avium sp. paratuberculosis (MAP) are the main members of MAC. MAH causes disseminated disease in immunocompromised patients, and MAP chronic enteritis in ruminants. Both mycobacteria are involved in a potential zoonotic role. Objective. To describe the genetic diversity of MAC causing disease in different host in Argentina. Materials and methods. It was included 18 MAH and 61 MAP isolates. MAH and MAP were isolated from human and cattle respectively. Human clinical samples were decontaminated by 2% NaOH and cultured on solid and liquid media. Samples from cattle were decontaminated by hexadecyl-pyridinium chloride and cultured on Herrold´s with mycobactin. Mycobacteria were first detected by direct smear examination by Ziehl-Neelsen stain and the species later identified by GenoType CM®. IS1311-PCR was performed to identify MAC, IS900-PCR to identify MAP and IS901-PCR to differentiate between MAH and MAA. The MIRU-VNTR loci 292, X3, 25, 47, 3, 7, 10 and 32 were used for genotyping. The patterns were assigned according with INMV database (http://mac-inmv.tours.inra.fr/). The allelic diversity (D) and the Hunter and Gaston discriminatory index (HGDI) were calculated by http://insilico.ehu.es/mini_tools/discriminatory_power/index.php. Results. Valid results were obtained in 71 (90%) isolates. Twelve (67%) MAH were correctly genotyped, 5 known patterns were found (INMV 121, n: 1; INMV 92, n: 2; INMV 97, n: 1; INMV 103, n: 1; INMV 50, n: 1) and other 5 no previously reported patterns appears among 6 isolates. The loci X3 and 292 had the highest D for MAH (0.7424 and 0.6515) and the global discriminatory power (HGDI) was 0.9697. Regarding MAP isolates, 59 (96.7%) showed results with 5 patterns (INMV 1, 42.4%; INMV 2, 28.8%; INMV 11, 20.3%; INMV 8, 5.1% and INMV 5, 3.4%). The global HGDI was 0.7043 and loci 292 and 7 showed the highest D (0.5 and 0.3). Discussion. Most of the isolates could be genotyped and no similar INMV patterns were found between MAP and MAH, showing MAH a higher diversity than MAP. The INMV, D and HGDI obtained with MAP are in concordance with those reported in Argentina but no previous reports exist for MAH isolates from human beings. Better knowledge of MAC diversity could improve the control and manage of these diseases on both human and animal sanitary systems.