IN A MOUSE MODEL OF DERMATITIS.

JULIETA ALCAIN ; GABRIELA SALAMONE; MONICA VERMEULEN; SOLEDAD GORI

Congreso; 63 Reunión Científica Anual de la Sociedad Argentina de Inmunol; 2016

Dermatitis is an inflammatory disease characterized by an imbalance of the immune response toward a Th2 profile, whose main inflammatory mediator is histamine (HIS). HIS exerts its actions through four receptors (H1-H4). Previously, we demonstrated, using a model of pulmonary inflammation to OVA, that HIS is able to induce the recruitment of CD8+ T lymphocytes in the lung of mice. The purpose of the present work was to study the modulatory role of HIS in another inflammatory disease affecting the skin, contact dermatitis, and the role of H3 and H4 receptors. For this, we developed a mouse model of dermatitis using the irritating agent dinitrophenyl benzene (0.7% DNFB in acetone/ water). For this, 20 ìl of DNFB (or vehicle) was administered for 4 consecutive days on both ears, along with 20 ìl of a H3/H4 receptor antagonist (thioperamide, Thio, 10-7M) on the right ear. Seven days after the first administration, DNFB (0.2% in PBS) was administered intraperitoneally in all mice. Topical treatment of the injured zone with Thio partially reversed the symptoms of the disease associated with inflammation in the application area (Ct, 0.267 mm ± 0.033 mm; DNFB, 0.667 mm ± 0.044 mm; DNFB+Thio, 0.416 mm ± 0.072 mm. p