GLIOBLASTOMA CELL LINE AND ITS IMPACT ON THE IMMUNE RESPONSE

MARIELA MORENO; WALTER SCORDO; LUCIANA MOVERER; GABRIELA SALAMONE; ANTONELA ASAD ALCAIN; MÓNICA VERMEULEN ; MARÍA SOLEDAD GORI; JULIETA ALCAIN; MARIANELA CANDOLFI

Congreso; 63 Reunión Científica Anual de la Sociedad Argentina de Inmunol; 2016

Glioblastoma multiforme (GBM) is the deadliest and most common type of human primary brain tumor. This tumor is defined by the hallmark features of uncontrolled cellular proliferation, diffuse infiltration, propensity for necrosis, robust angiogenesis, intense resistance to apoptosis and genomic instability. Acetylcholine (ACh) is a neurotransmitter which can also modulates cell survival, proliferation and differentiation in neuronal and non-neuronal cells such as immune cells, which has been referred to as a ?nonneuronal cholinergic system?. The aim of this work is to elucidate the relevance of the non-neuronal cholinergic system in the interaction immune-GBM cells. Methods: Human U251 GBM cells were cocultured with human dendritic cells (DC) obtained from healthy adult volunteer. Mononuclear cells were isolated from buffy coats of healthy adult nonsmoker volunteer and CD14+ cells were then isolated by positive selection and then were cultured with GM-CSF and IL-4. The cells were cultered in the presence of cholinergic agonists(carbachol, muscarine and nicotine) and antagonists (atropine and mecamelamine) and evaluated DC maturation. Results: We found that coculture of dendritic cells with U251 cells in the presence of carbachol and nicotine treatment increased the release of IL-8 (P