IL-10 deficency protects renal damage in a murine model of hemolytic uremic syndrome.

PINEDA, GONZALO EZEQUIEL; FERNANDEZ BRANDO, ROMINA J; REARTE, BARBARA; ISTURIZ, MARTIN AMADEO; ALBA SOTO, CATALINA; PALERMO, MARINA SANDRA; RAMOS, MARIA VICTORIA

Buenos Aires

Congreso; IV LASID Meeting. LXIII Argentinean Immunology Society Meeting; 2015

IL-10 deficency protects renal damage in a murine model of hemolytic uremic syndrome.Gonzalo Ezequiel Pineda1, Romina Jimena Fernandez-Brando1, Barbara Rearte2, Martin Isturiz2, Catalina Alba-Soto3, Marina Palermo1, Maria Victoria Ramos1.1Laboratorio de Patogenesis e Inmunologia de Procesos Infecciosos, IMEX-Academia Nacional de Medicina.2Laboratorio de Fisiologia de los procesos Inflamatorios, IMEX-Academia Nacional de Medicina.3Instituto de Investigaciones en Microbiología y Parasitología Médicas (IMPaM). Facultad de Medicina. Universidad de Buenos Aires.Background: Hemolytic Uremic Syndrome (HUS), a vascular disease characterized by nephropathy, is caused by enterohemorraghic-producing-Shiga-toxins (Stx) bacteria. The aim of the work was to determine the role of IL-10 in HUS evolution. Methods: Mice lacking IL-10 (IL-10-/-) and control mice were injected i.v. with 1LD100 Stx2. Before and after 2, 48 and 72h Stx2, mice were bled to perform polymorphonuclear leukocytes (PMN) counts, urea and cytokine evaluation. Results: IL-10-/- mice had a higher survival rate to Stx2 than controls (%survivors:44 and 0, median survival: 120h and 96h, respectively, *p