IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
B. abortus RNA through EGF-like ligands induces the retention of MHC-I molecules within the Golgi apparatus in human monocytes.
Autor/es:
MILILLO, M. AYELÉN; VELÁSQUEZ, LIS N.; DELPINO, M. VICTORIA; TROTTA, ALDANA; POZNER, ROBERTO G.; ISTURIZ, MARTÍN A.; GIAMBARTOLOMEI, GUILLERMO H.; BARRIONUEVO, PAULA
Lugar:
Buenos Aires
Reunión:
Congreso; LXIII Reunión Anual de la Sociedad Argentina de Inmunología. II Meeting Franco Argentino de Inmunología. IV Reunión de la Sociedad Latinoamericana de Inmunodeficiencias.; 2015
Institución organizadora:
Sociedad Argentina de Inmunología (SAI)
Resumen:
Despite the cytotoxic CD8+ T cell responses elicited by Brucella abortus, this intracellular pathogen is capable of surviving inside macrophages establishing a chronic infection. We recently reported that B. abortus RNA, described as a vita-PAMP, is able to down-modulate the IFN-γ-induced MHC-I expression on human monocytes. Also, we demonstrated that B. abortus RNA mimics the intracellular retention of MHC-I within the Golgi apparatus observed with the infection. The aim of this study was to further characterize the component and pathways involved in MHC-I down-modulation. For this, RNases-treated B. abortus RNA was employed to stimulate human monocytic THP-1 cells in the presence of IFN-γ for 48 h. Then, the expression of MHC-I molecules was evaluated by flow cytometry. Surprisingly, completely degraded RNA was able to inhibit MHC-I expression to the same extent as intact RNA (p