Dendritic cells internalize the foot-and-mouth disease virus involving LC3 autophagy and lysosomal LAMP proteins.

CECILIA LANGELLOTTI; JULIETA ALCAIN; IVANA SORIA; MARIELA GAMMELLA; VALERIA QUATTROCCHI; PATRICIA ZAMORANO; MÓNICA VERMEULEN

Medellín

Congreso; Inmunocolombia; 2015

ALAI

Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. This pathology is caused by foot-and-mouth disease virus (FMDV). Over time, the development of vaccines to prevent the spread of this illness became essential. Vaccines currently used contain the inactivated form of the virus (iFMDV). However, vaccination generates an immune response different to that induced by the infection. It was extensile demonstrated that FMDV interacts with autophagyc LC3 protein in certain cellular lines. However, the mechanisms involved on dendritic cells (DCs) entry are not well defined. We analyzed this fact on murine DCs. In the first place, the internalization of FMDV and iFMDV (moi:10) were confirmed by confocal microscopy. Then, we evaluated the expression of LC3 by western blot, and the expression of LC3 protein was increased in FMDV-infected cells, which was corroborated by flow cytometry (3-5% vs iFMDV; CT, p 0.05). Furthermore, we studied the proteins related lysosomes Lamp-1 and Lamp-2. Both Lamp-1 and Lamp-2 were significantly increased in DCs infected with FMDV. Notably, iFMDV was not associated to any of both lysosomal membranes. Interestingly, when we assessed late lysosomal vacuoles by confocal microscopy, we showed an important colocalization of FMDV with Lamp-2.As described, infectious virus in DCs was also associated to autophagy LC3 protein, and was found in lysosomal compartment; on the contrary, iFMDV was not associatedwith mature lysosomes.