IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Tear film hyperosmolarity breaks down the ocular surface?s immune tolerance: a role in initiating dry eye
Autor/es:
GUZMÁN, MAURICIO; KEITELMAN, IRENE; SABBIONE, FLORENCIA; TREVANI, ANALIA; GIORDANO, MIRTA N.; GALLETI, JEREMIAS G.
Lugar:
Denver
Reunión:
Congreso; ARVO annual meeting; 2015
Institución organizadora:
Association for research in vision and ophthalmology,
Resumen:
Purpose: Tear film hyperosmolarity is commonly observed in dry eye, but its exact role in the pathogenesis of this ocular surfacedisorder is unknown. As a localized form of autoimmune disease, dry eye involves disruption of the ocular surface s immune homeostasis by still uncharacterized stimuli. The purpose of this work was to evaluate whether hyperosmolar stress could affect conjunctival immune tolerance in mice.Methods: 8-to-12 week-old female Balb/c mice were instilled isoosmolar (0.3 Osm) or hyperosmolar (3 Osm) saline on both eyes 3 times daily for 5 days. Ovalbumin (OVA) was also instilled or OVA-pulsed dendritic cells (DCs) were subconjunctivally injected at different time points. Induced T cell responses were measured after subcutaneous immunization with OVA by the delayed-type hypersensitivity (DTH) assay. In addition, T cells in draining lymph nodes (day 5) were evaluated by flow cytometry. Supernatants from Pam212 epithelial cells exposed to iso- or hyperosmolar medium were assayed for cytokines or in a phagocytosis assay with the Raw 264.7 macrophage cell line.Results: Compared to non-instilled immunized mice, OVA-instilled mice developed reduced DTH responses (i.e. were tolerized), as did their OVA+0.3Osm saline-instilled cage mates (p