Interleukins plasma levels in HIV/HCV coinfected patients with or without antiretroviral therapy

BADANO MARÍA NOEL; PARODI CECILIA; ALOISI NATALIA; MONZANI CECILIA; CORTI MARCELO; NEME DANIELA; BRACCO MARÍA MARTA; BARÉ PATRICIA

Ciudad Autónoma de Buenos Aires

Congreso; I meeting LASID-FAIC-SAI; 2015

Latin American Society for Immunodeficiencies (LASID)

IL-10 and IL-2 plasma levels were analyzed by ELISA in a cohort of HIV/HCV-coinfected hemophilic patients. Patients were classified as those who received continuous antiretroviral therapy (ART) for more than 15 years, showing good adherence and response (G1, n=15) and those who did not initiate ART or showed poor adherence or no response (G2, n=16); 9 of which subsequently died. Samples from healthy donors (C1) and from non-infected hemophilic patients (C2) were used as controls. Statistical analysis was performed with Student t test and Mann Whitney.No significant differences were observed between G1 and controls in IL-2 (G1: 3.7; C1: 4.5; C2: 4.0 pg/ml) and IL-10 levels (G1: 6.9; C1: 7.5; C2: 6.2 pg/ml). However, within G1, IL-10 values were significantly lower compared to the pre-ART stages (10.7 vs 6.4 pg/ml; p=0.001). Instead, G2 showed significantly higher IL-2 (6.1 pg/ml) and IL-10 (14.1 pg/ml) levels than controls and G1 (p=0.01). As expected, G1 displayed higher CD4 counts and lower HIV viral load than G2 (not shown). Analyzing interleukins levels in relation to immunological and virological status, showed a negative correlation with CD4 counts (r=-0.5; p=0.01) and a positive correlation with viral load (r=0.5; p=0.01).Continuous and good adherence to ART is not only necessary to achieve viral control but it may also contribute to the immune restoration by reverting CD4 counts and cytokine production.Changes in interleukins plasma values deserve further investigation since the following of these biomarkers could be useful as predictors for clinical disease progression in HIV/HCV patients.