Neutrophil-Derived Circulating Free DNA (cf-DNA): an associated marker for Hemolytic Uremic Syndrome development.

RAMOS, MARIA VICTORIA; MEJIAS, PILAR; SABBIONE FLORENCIA; ABREY-RECALDE, MARIA JIMENA; FERNANDEZ BRANDO, ROMINA J; AMARAL, MARIA MARTA; EXENI R; TREVANI, ANALIA; PALERMO, MARINA SANDRA

Mar del Plata. Prov de Buenos Aires

Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXII Reunión Científica de la Sociedad Argentina de Inmunología (SAI); 2014

Sociedad Argentina de Inmunologia. Sociedad Argentina de Investigación Clinica

Neutrophil-Derived Circulating Free DNA (cf-DNA): an associated marker for Hemolytic Uremic Syndrome development. 1Ramos, MV; 1Mejias, MP; 1Sabbione, F; 1Abrey-Recalde, MJ; 1Fernandez-Brando,RJ; Amaral, MM; 3Exeni, R; 1Trevani, A; 1Palermo, MS. 1IMEX-CONICET, Academia Nacional de Medicina; 2Facultad de Medicina. UBA; 3Htal de Niños de San Justo Hemolytic Uremic Syndrome (HUS), a vascular disease characterized by hemolityc anemia, thrombocitopenia and acute renal failure is caused by enterohemorraghic Shiga toxins (Stx)-producing bacteria. Besides Stx, inflammatory response mediated by neutrophils (PMN) is essential to HUS evolution. PMN have the capacity to release "neutrophil extracellular traps" (NETs) which are involved in the pathogenesis of several diseases. NETs are composed of neutrophil-derived circulating free DNA (cf-DNA), histones and PMN proteins such as proteases. The aim of this work was to investigate the presence of cf-DNA in HUS patients. We observed increased values of cf-DNA in plasma from HUS compared to healthy children (HC) ((HC:5,1±0,5; HUS:10±0,5*)ug/ml, n=15, *p