Effect of glucose levels in increased neutrophil extracelular trap formation (NETs) in type 2 diabetes patients at diagnosis and after 6 and 12 months of metabolic control.

SCHATTNER, M; CARESTIA, A; FRECHTEL, G; CERRONE, G; GONZALEZ C; LINARI, MA; CASAIS, P

Toronto

Congreso; XXV Congress of the International Society on Thrombosis and Haemostasis (ISTH); 2015

Effect of glucose levels in increased neutrophil extracelular trap formation (NETs) in type 2 diabetes patients at diagnosis and after 6 and 12 months of metabolic control. Mirta Schattner1, Agostina Carestia1, Gustavo Frechtel2, Gloria Cerrone2, Claudio Gonzalez3, and Patricia Casais1.1Laboratory of Experimental Thrombosis, Institute of Experimental Medicine-CONICET-National Academy of Medicine, Buenos Aires, Argentina.2Genetics and Molecular Biology, Department of Microbiology, Immunology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires (UBA), Buenos Aires, Argentina.3Department of Pharmacology, School of Medicine, University of Buenos Aires (UBA), Buenos Aires, Argentina. Diabetes is characterized by a chronic inflammatory state, endothelial dysfunction, an increased risk of infections and early cardiovascular disease. The release of DNA-derived neutrophil extracellular traps (NETs) is a mechanism by which these cells in concerted action with platelets and endothelium kill bacteria and exert proinflammatory and prothrombotic activities. The aim of this study was to determine the levels of nucleosomes in diabetic patients, whether the ability of their neutrophils to form DNA traps differs from healthy donors and their relationship with inflammatory biomarkers.Recently diagnosed diabetic patients (12, glycaemia 210±29 mg/dl) and healthy donors (12) were included. NET formation was studied by microscopy and using Syber Gold. Plasma levels of nucleosomes and von Willebrand factor (vWF) were measured by ELISA and P-selectin expression, by cytometry. Microscopy and quantification of free DNA studies showed that while neutrophils from healthy donors did not form NETs without stimulation, patients exhibited spontaneous NET formation (control:0.2±0.04 vs. patients:0.5±0.1 g/ml of DNA,p