IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
REQUIREMENT OF AUTOPHAGY FOR IL-1β SECRETION IN HUMAN
Autor/es:
IULA, LEONARDO; KEITELMAN, IRENE; SABBIONE, FLORENCIA; JANCIC, CAROLINA; TREVANI, ANALIA
Lugar:
Mar del Plata
Reunión:
Congreso; LXII REUNIÓN ANUAL SAI-SAIC; 2014
Institución organizadora:
Sociedad Argentina de Investigacion Clinica
Resumen:
Interleukin-1β (IL-1β) is a pro-inflammatory cytokine synthesized in the cytoplasm as a precursor, pro-IL-1β, which has to be proteolytically processed to acquire biological activity. In human neutrophils (PMN) IL-1β processing is dependent of caspase-1 and elastase and/or proteinase-3. The release of IL-1β does not follow the conventional ER-Golgi route of secretion, being secreted by poorly-defined non-conventional secretory pathways. The current study was undertaken to test the hypothesis that a non conventional autophagy (Au) mechanism is required for neutrophil IL-1β secretion. We have previously determined that the Au inhibitors 3-MA, wortmannin and Bafilomycin A1 inhibit IL-1β secretion induced by LPS and LPS+ATP. In this study we determined that in response to these agonists, the secretion of IL-8, a cytokine whose release follows the canonical ER-Golgi pathway, was not regulated by the above mention inhibitors. We also observed that Au stimulation by starvation promoted IL-1β secretion induced by both agonists (n=4; p