Leukotriene C4 increases the susceptibility of adult mice to Enterohemorragic Escherichia Coli infection

CABRERA, G; FERNANDEZ BRANDO, ROMINA J; MEJIAS, PILAR; RAMOS, MARIA VICTORIA; ABREY-RECALDE, MARIA JIMENA; ABREY-RECALDE, MARIA JIMENA; VANZULLI, SILVIA; VERMEULEN, M; PALERMO, MARINA SANDRA

Mar del Plata, Prov Buenos Aires, Argentina

Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXII Reunión Científica de la Sociedad Argentina de Inmunología (SAI); 2014

Sociedad Argentina de Inmunología - Sociedad Argentina de Investigacion Clinica

Leukotriene C4 increases the susceptibility of adult mice to Enterohemorragic Escherichia Coli infection Gabriel Cabrera, Romina J. Fernández-Brando, María Pilar Mejías, María Victoria Ramos, María Jimena Abrey-Recalde, Silvia Vanzulli, Mónica Vermeulen and Marina S. Palermo. Shiga-toxin (Stx) producing Escherichia coli (EHEC) strains can cause a broad spectrum of human diseases, including the life-threatening hemolytic uremic syndrome (HUS). Despite EHEC infection is characterized by acute inflammation of the colonic mucosa, little is known regarding the role of proinflammatory mediators like cysteine leukotrienes (LTC4) in this pathology. Thus, the aim of this work was to analyze whether LTC4 influence EHEC pathogenesis in mice. BALB/c mice were pretreated with exogenous LTC4 (0,01uM) before EHEC intragastric infection (5-7x1011 CFU of EHEC/Kg of body weight). LTC4-treated (LTC4+) EHEC-infected (EHEC+) mice showed the highest intestinal damage, as assessed by histology, displaying submucosal infiltration and the highest number of apoptotic epithelial cells. LTC4+/EHEC+ mice showed a decreased survival rate as compared to LTC4-non-treated (LTC4-) EHEC+ mice. Survival: LTC4+/EHEC+= 62,5%, n=16; LTC4-/EHEC+=93,7% n=16, p