IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IMPACT OF M. tuberculosis GENETIC VARIABILITY ON THE HUMAN BRONCHIAL EPITHELIUM RESPONSE
Autor/es:
DENISE KVIATCOVSKY; LUCIANA BALBOA; PABLO SCHIERLOH; BEATRIZ LÓPEZ; MARÍA DEL CARMEN SASIAIN; SILVIA DE LA BARRERA
Lugar:
Aguascalientes
Reunión:
Taller; IV Curso Internacional: Inmunidad Innata contra Patógenos; 2014
Institución organizadora:
Universidad Autónoma de Aguascalientes
Resumen:
Mycobacterium tuberculosis (Mtb) infects primarily the lung and is recognized for its ability to evade innate and adaptive host responses. The respiratory epithelium not only acts as a mechanical barrier for gaseous exchange, but also as an immune sentinel. Recent evidence demonstrates that Mtb genotypes would modulate the immune response through different evasion mechanisms. We have shown that the outbreak multidrug-resistant strains of Mtb, M and Ra, differentially modulate in vitro innate and adaptive immune response in humans. The question then arises whether M and Ra strains are able to activate differentially the bronchial epithelium. We have previously demonstrated that the direct interaction between different Mtb strains and bronchial epithelial cells (Calu-6) did not result in the modulation of the phenotype (e.g. TLR-2, CD54 and CD11b expression) or the induction of cell death in Calu-6. Moreover, Mtb strains showed a poor ability to invade the epithelium. However, we did observe a bystander effect from Mtb-stimulated macrophages (Mac) on Calu-6. H37Rv- and Ra-stimulated Mac were able to up-regulate CD54 in Calu-6, while M-stimulated Mac did not. In function of these results, we propose to identify the mechanism whereby M strain is not efficient enough to trigger the bystander effect on Calu-6.